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KMID : 0380620080400020190
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Korean Journal of Food Science and Technology
2008 Volume.40 No. 2 p.190 ~ p.193
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Engineering/Processing/Sensory Evaluation : Optimization of Enzymatic Hydrolysis for the Production of Antitoxic Bovine Hepatic Extract
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Kim Hyun-Jung
Kwon Do-Hyeong
Shon Dong-Hwa
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Abstract
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Bovine hepatic extract is recognized as possessing detoxifying activity against various liver diseases. In order to develop a process for its mass production, various enzymatic hydrolysis conditions were tested, and bovine hepatic extracts were prepared. These extracts were then examined for composition, microorganism levels, and vitamin B12 content. Among the enzymes tested, papain was selected based on yields for dry residue and amino nitrogen. The other enzymes tested included bromelain, ficin, pancreatin, and protease NP. The optimal hydrolysis conditions were established at 65℃ for 24 hr, with an addition of 1%(w/w) papain to the beef liver. The prepared spray-dried bovine hepatic extract showed an 11% recovery yield on a raw beef liver basis, with 95% dry residue and 11.8% total nitrogen content. Microorganisms were not detected in the dried extract, and its vitamin B12 content was 4.1 μg/g. In summary, the conditions established in this study could be applied for the high yield mass production of bovine hepatic extract.
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KEYWORD
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bovine hepatic extract, papain, hydrolysis, optimization
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